Identification of multiple chromosome 12 abnormalities in human testicular germ cell tumors by two-color fluorescence in situ hybridization (FISH)

✍ Scribed by Teresa A. Smolarek; Ruthann I. Blough; Richard S. Foster; Thomas M. Ulbright; Catherine G. Palmer; Nyla A. Heerema

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 556 KB
• Volume: 14
• Category: Article
• ISSN: 1045-2257
• DOI: 10.1002/gcc.2870140403

✦ Synopsis

The distribution of segments of the short and long arms of chromosome I 2 was distinguished by two-color fluorescence in situ hybridization (FISH) in 27 cytogenetically abnormal testicular germ cell tumors (TGCTs). A I 2p-specific probe was developed by chromosomal microdissection and sequence-independent polymerase chain reaction (PCR) amplification and was combined with a commercially available whole-chromosome I 2 painting probe. The TGCTs included both i( I2p)-positive and i( I2p)negative primary tumors and lymph node metastases from patients in clinical stage I or stage II who were not previously treated with chemotherapy. Rearrangements of the short arm of chromosome I 2 and overrepresentation of I2p DNA sequences were found in all cases. In addition, cryptic rearrangements of I2p were found in 39% (7/ 18) of the i( I 2p)-positive tumors and in 78% (7/9) of the i( I 2p)-negative tumors. Only 7% (2/27) of all tumors had cryptic rearrangements of I2q. Genes Chrornosorn Cancer /4:252-258 (199.5).