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Identification of Multi-Residue Motifs in Short Sequence Fragments of Membrane Protein

✍ Scribed by Lin, Meishan

Book ID
122292924
Publisher
Biophysical Society
Year
2011
Tongue
English
Weight
37 KB
Volume
100
Category
Article
ISSN
0006-3495

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A procedure is described for the in situ carboxamidomethylation of cystine/cysteine residues in protein samples of as little as \(10 \mathrm{pmol}\), prior to automated protein sequence analysis. Previous in situ methods for the modification of cysteines are limited to proteins available in quantiti