Identification of mineralized elastic fibers on wet samples by SEM
✍ Scribed by Federica Boraldi; Massimo Tonelli; Dealba Gheduzzi; Ivonne Pasquali Ronchetti; Daniela Quaglino
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 256 KB
- Volume
- 67
- Category
- Article
- ISSN
- 1059-910X
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✦ Synopsis
Abstract
A method is described that could be of potential use for the rapid ultrastructural identification of abnormal and fragmented elastic fibers in very small wet samples of dermal biopsies from patients affected by Pseudoxanthoma elasticum (PXE). Moreover, the method, which consists of the use of sealed capsules transparent to electrons, allows the rapid and accurate localization and detection of mineralized areas in PXE patients and of their ion composition by X‐ray microanalysis. This methodology could be of great help in any tissue disorder, especially in connective tissue disorders, characterized by structural alterations associated with ion precipitation. Microsc. Res. Tech. 67:296–299, 2005. © 2005 Wiley‐Liss, Inc.