Identification of microsatellite markers in the rye genome

✍ Scribed by Hanna Bolibok; Monika Rakoczy-Trojanowska; Małgorzata Wyrzykowska; Magdalena Radecka; Wacław Orczyk

• Book ID: 111490314
• Publisher: SP Versita
• Year: 2006
• Tongue: English
• Weight: 320 KB
• Volume: 11
• Category: Article
• ISSN: 1425-8153
• DOI: 10.2478/s11658-006-0023-5

✦ Synopsis

Abstract

The rye genomic library, which consists of DNA fragments in the range of 0.5–1.1 kb, was screened for the presence of tri-and tetranucleotide and compound microsatellites. Of the 1,600,000 clones analysed, 102 clones were positive and 41 were suitable for SSR primer pair design. Twenty-six primer pairs amplified specific products, and six of them were capable of detecting polymorphism among 30 rye accessions of different genetic backgrounds. Using a set of Chinese Spring-Imperial wheat-rye addition lines, it was possible to locate 3 newly identified microsatellites on chromosomes 3R, 4R and 7R.