Identification of macrophages in the muscle biopsy preparations: A comparative study using specific monoclonal antimacrophage antibodies and acid phosphatase reaction
✍ Scribed by Alexandros A. Drosos; Marinos C. Dalakas
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 1009 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0148-639X
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✦ Synopsis
Macrophages belong to the mononuclear phagocytic system, a cell lineage originating from the bone marrow. They play an important role in mediating muscle fiber injury in patients with inflammatory myopathies and muscular dystrophies, serving as antigen presenting cells, phagocytic cells, or cytotoxic cells. In tissues, macrophages can be identified on the basis of morphological characteristics, phagocytic properties, or expression o f specific surface molecules. 1 4 , 1 5
In muscle enzyme histochemistry, the most widely used method for identifying macrophages associated with nonnecrotic, necrotic, or degenerating muscle fibers is the acid phosphatase (AP) reaction which is based on increased lysosomal activity within the phagocytic cell^.^,'^^," It is not clear, however, if AP recognizes only the macrophages associated with phagocytosis or all the phagocytic cells including the functionally spectic subsets that serve as primary immune effector cells. The availability of monoclonal antibodies that identify antigenic markers on macrophages, according to different stages of maturation or function, 1 ,g, 12 offer the opportunity to investigate the number and type of macrophages recognized by the AP reaction. In the present study we compared