## Abstract Immunoreactive luteinizing hormone releasing hormone (irLHRH) and immunoreactive gonadotropin (irGtH) are demonstrated in the pituitary gland of a teleost fish (__Xiphophorus maculatus__, the platyfish) at the ultrastructural level. Using an immunoperoxidase procedure, irGtH and irLHRH
Identification of luteinizing hormone-releasing hormone (LH-RH) in the brain and pituitary gland of a fish by immunocytochemistry
✍ Scribed by Schreibman, M. P. ;Halpern, L. R. ;Th. Goos, H. J. ;Margolis-Kazan, H.
- Publisher
- John Wiley and Sons
- Year
- 1979
- Tongue
- English
- Weight
- 366 KB
- Volume
- 210
- Category
- Article
- ISSN
- 0022-104X
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✦ Synopsis
Abstract
For the first time immunoreactive luteinizing hormone‐releasing hormone (LH‐RH) is demonstrated in both the brain and pituitary gland of a teleost (Xiphophorus maculatus) using an immunoperoxidase procedure. It is specifically localized in the perikarya and their axons of the ventral telencephalon and nucleus lateralis tuberis and within and between the gonadotrops and within some cells of the pars intermedia. These immuno‐reactions are extinguished when antiserum to LH‐RH is preincubated with LH‐RH antigen but not with neurohypophysial hormones.
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## Abstract The immunoperoxidase technique was used to identify luteinizing hormone‐releasing factor (LRF) and the alpha subunit of glycoprotein hormones in ductal carcinoma of the mammary gland. Eleven tumors were studied, using antisera against LRF and alpha subunit, and against the beta subunits