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Identification of intermediates of in vivo trichloroethylene oxidation by the membrane-associated methane monooxygenase

โœ Scribed by Sonny Lontoh; James A. Zahn; Alan A. DiSpirito; Jeremy D. Semrau

Book ID
117516334
Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
218 KB
Volume
186
Category
Article
ISSN
0378-1097

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Methylosinus trichosporium OB3b biosynthesizes a broad specificity soluble methane monooxygenase that rapidly oxidizes trichloroethylene (TCE). The selective expression of the soluble methane monooxygenase was followed in vivo by a rapid colorimetric assay. Naphthalene was oxidized by purified solub