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Identification of integrated hepatitis B virus DNA and expression of viral RNA in an HBsAg-producing human hepatocellular carcinoma cell line

โœ Scribed by Chakraborty, Prasanta R.; Ruiz-Opazo, Nelson; Shouval, Daniel; Shafritz, David A.


Book ID
109719285
Publisher
Nature Publishing Group
Year
1980
Tongue
English
Weight
441 KB
Volume
286
Category
Article
ISSN
0028-0836

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Identification of integrated hepatitis B
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DNA extracts from hepatocellular carcinomas of 13 patients from South Africa were examined for hepatitis B virus (HBV) DNA sequences by molecular hybridization using [32P]-labeled recombinant, cloned, and purified HBV-DNA. Eight patients were HBV carriers as demonstrated by the presence of hepatitis

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Integration of hepatitis B virus (HBV) DNA into host DNA is detected in about 90% of HBV-related hepatocellular carcinoma (HCC), but the preferential sites of the viral integration etiologically relevant to oncogenesis have been controversial. By using an adaptor-ligation/suppression-PCR, we identif