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Identification of gibberellin A20, abscisic acid, and phaseic acid from floweringBryophyllum daigremontianumby combined gas chromatography-mass spectrometry

✍ Scribed by P. Gaskin; J. MacMillan; J. A. D. Zeevaart

Publisher: Springer-Verlag
Year: 1973
Tongue: English
Weight: 345 KB
Volume: 111
Category: Article
ISSN: 0032-0935
DOI: 10.1007/bf00385554

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✦ Synopsis

The presence of abscisic and phaseic acid in a purified acidic extract from flowering plants of the long-short-day plant Bryophyllum daigremontianum [(R. Hamet and Perr.) Berg.] was conclusively established by combined gas chromatography-mass spectrometry (GC-MS) of their methyl esters. Gibberellin A20 (GA20) was identified by GC-MS of the methyl ester and the trimethylsilyl ether of the methyl ester. The following levels of the 3 compounds per kg fresh weight were estimated: Abscisic acid, 5.5 μg; phaseic acid, 9.4μg; gibberellin A20, 0.8 μg. When GA20 and four other GAs were applied to Bryophyllum under shortday conditions, the order of effectiveness for induction of flower formation was: GA2>GA1>GA5=GA7>GA20. The low biological activity of the native GA20 is discussed.

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