Identification of Escherichia coli proteins cross-reacting with antibodies against region 2.2 peptide of RNA polymerase sigma subunit

Elution of proteins from sodium dodecyl

Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: Results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes

✍ Dayle A. Hager; Richard R. Burgess 📂 Article 📅 1980 🏛 Elsevier Science 🌐 English ⚖ 965 KB

tained from Worthington (Freehold, N. J.). Wheat germ DNA topoisomerase was purified in our laboratory according to a procedure to be published elsewhere (W. Dynan. J. Jendrisak. D. Hager. and R. Burgess, unpublished procedure). Wheat cu-amylase was provided by R. Boston and plasmid pBR322 DNA was t