Identification of disease causing loci using an array-based genotyping approach on pooled DNA

Highly sensitive DNA detection and point

Highly sensitive DNA detection and point mutation identification: an electrochemical approach based on the combined use of ligase and reverse molecular beacon

✍ Zai-Sheng Wu; Jian-Hui Jiang; Guo-Li Shen; Ru-Qin Yu 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 279 KB

A novel strategy is described for highly sensitive DNA detection and point mutation identification based on the combination of reverse molecular beacon with DNA ligase. A 5'-phosphoryl and 3'-ferrocene terminated DNA sequence is used as detection probe, which may be ligated to capture DNA immobilize