Identification of cytomegalovirus in a liquid-based gynecologic sample using morphology, immunohistochemistry, and DNA real-time PCR detection
✍ Scribed by Harmanjatinder S. Sekhon; Richard D. Press; Waldemar A. Schmidt; Mary Hawley; Anne Rader
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 322 KB
- Volume
- 30
- Category
- Article
- ISSN
- 8755-1039
- DOI
- 10.1002/dc.20048
No coin nor oath required. For personal study only.
✦ Synopsis
The viral cytopathic effects of cytomegalovirus (CMV) are rarely encountered in conventional cervical vaginal smears and have never been reported in a liquid-based gynecologic sample (SurePath). We present results of a liquid-based gynecologic sample (SurePath) from an asymptomatic patient with classic CMV-associated granular or dense cyanophilic intracytoplasmic and intranuclear inclusion bodies with a clear surrounding zone. These inclusions were immunohistochemically positive for CMV. The patient also had human papilloma virus (HPV)-associated koilocytes that showed a unique perinuclear immunostaining pattern suggesting coinfection with both CMV and HPV. CMV amplification using real-time polymerase chain reaction (PCR) of DNA extracted from the liquid-based sample confirmed the morphologic and immunohistochemical findings of CMV infection. These observations suggest that a liquid-based preparation can be used to assess CMV infection morphologically, immunohistochemically, and by real-time PCR.