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Identification of collagen types in tissues using HPLC-MS/MS

✍ Scribed by Statis Pataridis; Adam Eckhardt; Katerina Mikulíková; Pavla Sedláková; Ivan Mikšík

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
374 KB
Volume
31
Category
Article
ISSN
1615-9306

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

A method for the determination and quantification of collagen types I–V in rat tissues has been developed. This method is based on collagen fragmentation by cyanogen bromide followed by trypsin digestion. After that, HPLC‐MS/MS (HPLC coupled to an IT mass spectrometer) analyses of the resulting peptide mixtures (peptide maps) were performed. Specific peptides for each collagen type were selected. According to online databases, these peptides are present in human, bovine, and rat collagens. As a result, this method can be potentially applied to other species' tissues as well, such as human tissues, and provides a universal and simple method of quantifying collagen types. The applicability of this method for analyzing collagen types was demonstrated on rat tissues (skin, tendon, and aorta).

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