Abstract
In colorectal cancer (CRC) care, treatment decisions depend on the efforts to estimate the metastatic potential of tumors. The liver is one of the most common metastatic sites of CRC and the prognosis of CRC patients often reflects metastases to distant sites. To identify chromosomal aberrations associated with liver metastasis, we performed allelic copy number analysis for CRC with or without synchronous liver metastasis using genotyping arrays. By allelic copy number analysis of CRC samples, we observed common aberrations in 14 chromosomal arms in two groups, that is, gains on 7p22.3βp11.2, 8q22.3βq24.3, 13q12.12βq34, and 20q11.22βq13.33 and loss of heterozygosity (LOH) on 4q12βq35.1, 5q11.2βq35.3, 8p23.3βp12, 15q11.2βq26.3, 17p13.3βp11.2, 17q11.2βq25.1, 18p11.32βp11.21, 18q11.2βq23, 20p13βp12.1, and 22q11.1βq13.32. We found that gains on 20p13βp12.1 and 20q11.21βq13.33 and LOH on 6q14.1βq25.1 were more frequent in CRC with liver metastasis. We also compared chromosomal aberrations in primary CRC lesions with those of the corresponding liver metastasis and found that the allelic genome imbalance status of a metastatic lesion is similar to that of the primary cancer, which suggests that chromosomal aberrations are largely maintained on hematogenous spread. Intriguingly, several chromosomal aberrations in CRC were found in the primary cancer but not in the corresponding liver metastasis, thus suggesting heterogeneity of cancer cells within solid tumors or the presence of events uniquely developed in primary tumors. Consequently, CRC with and without liver metastasis harbor similar chromosomal aberrations, and chromosomal aberration at 6q, 20p, and 20q may be involved in the process of liver metastasis of CRC. Β© 2010 WileyβLiss, Inc.