Identification of carcinogenic tryptophan pyrolysis products in human bile by high-performance liquid chromatography

✍ Scribed by Dr. Shigeo Manabe; Osamu Wada

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 608 KB
• Volume: 15
• Category: Article
• ISSN: 0893-6692
• DOI: 10.1002/em.2850150410

✦ Synopsis

The carcinogenic tryptophan pyrolysis products 3-amino-l,4-dimethyl-5H-pyrido [4,3-b] indole (Trp-P-l ) and 3-amino-1 -methyl-5H-pyrido[4,3blindole (Trp-P-2) have been demonstrated to be present in human bile through use of a highperformance liquid chromatography (HPLC) method. The method consists of the acidinduced release of the carcinogens from bile components and their extraction with methylene chloride and subsequent quantification by HPLC. In seven subjects who had received cath-eterization of the bile duct and external biliarydrainage, the average amounts of Trp-P-1 and Trp-P-2 excreted daily in the bile were 408 fmol/ day (n = 7) and 864 fmol/day (n = 7), respectively. In one subject, furthermore, significant daily changes of these carcinogen levels in bile and plasma were confirmed during 2 weeks of observation. These results indicate that one of the excretory pathways of these carcinogens is via bile. Our data also may suggest that Trp-P-1 and Trp-P-2 are derived from everyday foods.