Cloning, expression, and crystallization
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Kyeong Kyu Kim; Hisao Yokota; Rosalind Kim; Sung-Hou Kim
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Article
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2008
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Cold Spring Harbor Laboratory Press
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English
⚖ 266 KB
## Abstract A gene coding for a protein homologous to a translation initiation factor of eukaryotes, eIF5A, was cloned from __Methanococcus jannaschii__, a hyperthermophile with an optimum growth temperature of 85 °C. The protein was overexpressed, purified and crystallized. The crystals were obtai