Identification of amplified restriction fragment polymorphism (AFLP) markers tightly linked to the tomato Cf-9 gene for resistance to Cladosporium fulvum

✍ Scribed by Colwyn M. Thomas; Pieter Vos; Marc Zabeau; David A. Jones; Karen A. Norcott; Brian P. Chadwick; Jonathan D.G. Jones

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 921 KB
• Volume: 8
• Category: Article
• ISSN: 0960-7412
• DOI: 10.1046/j.1365-313x.1995.08050785.x

✦ Synopsis

Using the technique of amplified restriction fragment polymorphism (AFLP) analysis, and bulked segregant pools from F~2~ progeny of the cross Lycopersicon esculentum (Cf9)×L. pennellii, approximately 42 000 AFLP loci for tight linkage to the tomato Cf‐9 gene for resistance to Cladosporium fulvum have been screened. Analysis of F~2~ recombinants identified three markers which co‐segregated with Cf‐9. The Cf‐9 gene has recently been isolated by transposon tagging using the maize transposon Dissociation (Ds). Analysis of plasmid clones containing Cf‐9 shows that two of these markers are located on opposite sides of the gene separated by 15.5 kbp of intervening DNA. AFLP analysis provides a rapid and efficient technique for detecting large numbers of DNA markers and should expedite plant gene isolation by positional cloning and the construction of high‐density molecular linkage maps of plant genomes.