Identification of aminophospholipid stereomers by positive-ion fast atom bombardment combined with collisional activation mass-analysed ion kinetic energy analysis and high-performance liquid chromatography

✍ Scribed by Su Chen; Giampaolo Menon; Pietro Traldi

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 330 KB
• Volume: 27
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/oms.1210270311

✦ Synopsis

Two methods for the identification of aminophospholipid stereomen are described. After a chemical derivatization, 9-fluorenylmethoxycarbonyl derivatives of diacyl-sn-2-and diacyl-sn-Sphosphoserine and diacyl-sn-2-and diacylsn-3-phosphoethanolamine can be characterized by positive-ion fast atom bombardment combined with colliiional activation mass-analysed ion kinetic energy analysis based on the differentiation of relative abundances [ M + H + diethanolamine -891 + and [ M + H] + fragments derived from (M + H + diethanolamine] + ions, the protonated solvated molecules, and normal phase high-performance liquid chromatography on the basis of different elution times of the derivatives of the aminophospholipid stereomers on an aminopropyl-bonded column.