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Identification of amino acid residues important for the phosphomannose isomerase activity of PslB in Pseudomonas aeruginosa PAO1

โœ Scribed by Hui-Ju Lee; Hwan-You Chang; Nandinin Venkatesan; Hwei-Ling Peng


Book ID
113620991
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
482 KB
Volume
582
Category
Article
ISSN
0014-5793

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The gene braB, encoding the Na+-coupled carrier for branched-chain amino acids in Pseudomonas aerugin- osa PAO, was cloned on cosmid pMMB34. The cosmid clones carrying the braB gene were identified as those that restored growth at low leucine concentration and Na+-dependent leucine transport activit