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Identification of a yeast artificial chromosome spanning the 8q12 translocation breakpoint in pleomorphic adenomas with t(3;8)(p21;q12)

✍ Scribed by Eva Röijer; Koen Kas; Ingrid Klawitz; Jörn Bullerdiek; Wim Van de Ven; Göran Stenman

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
569 KB
Volume
17
Category
Article
ISSN
1045-2257

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✦ Synopsis

A subgroup of pleomorphic adenomas of the salivary glands is characterized by translocations involving chromosome 8, with consistent breakpoints at 8q 12. As part of a positional cloning effort to isolate the gene(s) affected by these translocations we now report the mapping of the 8q I 2 breakpoint in two primary pleomorphic adenomas with the recurrent t(3;8)(p2I ;q 12). Yeast artificial chromosome (YAC) clones corresponding to eight different loci in 8ql1-12 were isolated and mapped by fluorescence in situ hybridization (FISH). The t(3;8) breakpoint was mapped within a I Mb region flanked by MOS proximally and by the genetic marker D8S I66 distally. One YAC within this region was shown to span the t(3;8) breakpoint in two tumors. This YAC will provide an excellent tool for isolating the gene@) at the breakpoint(@ in adenomas with t(3;8).

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