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Identification of a whole-arm translocation by in situ hybridization with directly fluorochrome-labeled probes in a myelodysplastic syndrome

✍ Scribed by Svetlana Bajalica; Dr. Karen Brøindum-Nielsen; Anne-Grethe Sørensen; Niels Tinggaård Pedersen; Sverre Heim

Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
408 KB
Volume
5
Category
Article
ISSN
1045-2257

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✦ Synopsis

A case of myelodysplasia was found to have a complex bone marrow karyotype, involving an apparent whole-arm translocation between 17q and 18q. The application of a simplified fluorescence in situ hybridization technique, using directly fluorochromelabeled centromere-specific alpha-satellite DNA probes, demonstrated the presence of sequences from both chromosomes 17 and I8 in the centromere of the derivative chromosome. This proves that a true whole-arm translocation had occurred.

The case exemplifies how in situ hybridization analysis can be used to resolve interpretation problems in cancer cytogenetics.

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