Fluorescence Assay for the Binding of Ri
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Richele L. Abel; Marcia C. Haigis; Chiwook Park; Ronald T. Raines
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Article
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2002
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Elsevier Science
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English
β 222 KB
Ribonuclease A (RNase A) and the ribonuclease inhibitor protein (RI) form one of the tightest known protein-protein complexes. RNase A variants and homologues, such as G88R RNase A, that retain ribonucleolytic activity in the presence of RI are toxic to cancer cells. Herein, a new and facile assay i