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Identification of a putative methylenetetrahydrofolate reductase by sequence analysis of a 6·8 kb DNA fragment of yeast chromosome VII

✍ Scribed by Tizon, Belen; Rodríguez-Torres, Ana M.; Rodríguez-Belmonte, Esther; Cadahia, Jose L.; Cerdan, Esperanza

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
390 KB
Volume
12
Category
Article
ISSN
0749-503X

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✦ Synopsis

We report the sequence analysis of a 6.8 kb DNA fragment from Saccharomyces cerevisiue chromosome VII. This sequence contains five open reading frames (ORFs) greater than 100 amino acids. There is also an incomplete O R F flanking one of the extremes, G2868, which is the 3' end of the SCS3 gene (Hosaka et d., 1994). The translated sequence of O R F G2882 shows similarity to the human methylenetetrahydrofolate reductase (Goyette ef al., 1994). O R F (32889 shows no significant homologies with the sequences compiled in databases. ORF G2893 corresponds to the gene SUP44, coding for the yeast ribosomal protein S4 (All-Robin et al., 1990). G2873 and (32896 are internal ORFs, !The whole sequence of the fragment is available at the EMBL nucleotide sequence database, 'GenBank and Data Bank of Japan under the Accession Number X94106.

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