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Identification of a polymorphism in intron 2 of the p53 gene

โœ Scribed by Lisa M. Pleasants; Marc F. Hansen

Publisher
Springer
Year
1994
Tongue
English
Weight
238 KB
Volume
93
Category
Article
ISSN
0340-6717

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โœฆ Synopsis

A p o l y m o r p h i s m in intron 2 of the p53 gene was identified by single-strand conformation polymorphism analysis. The result is a G-to-C transversion at bp +38 following the splice donor site of exon 2.

PCR sequencing

SSCP results of all individuals were confirmed by direct PCR sequencing using ATaq Cycle Sequencing System (United States Biochemical Corporation).

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