Identification of a phylogenetically conserved Sug1 CAD family member that is differentially expressed in the mouse nervous system
✍ Scribed by Sun, Danhui ;Swaffield, Jonathan C. ;Johnston, Stephen Albert ;Milligan, Carolanne E. ;Zoeller, R. Thomas ;Schwartz, Lawrence M.
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 378 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0022-3034
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✦ Synopsis
We have isolated a cDNA clone mouse brain by in situ hybridization. While all three from mouse, m56, that encodes a member of the Congenes had similar patterns of expression, there were served ATPase-containing Domain (CAD) protein significant differences among them. In moths, the exfamily. Sequence analysis revealed that m56 is identipression of the Sug1 homolog 18-56 is dramatically cal to mouse mSug1/FZA-B and shares high homolup-regulated during programmed cell death. In addiogy with human Trip1, moth 18-56, and yeast Sug1.
tion, it has been previously demonstrated that the pro-When examined, Sug1-like CAD proteins appear to teasome plays an essential role in the regulation of function in the regulation of the 26S proteasome, as apoptosis in mammals. We examined the expression well as associate with members of the steroid/thyroid of m56 in mouse during natural and induced cell death receptor superfamily and other transcriptional activain a variety of tissues and found no significant changes tors. m56 can complement the lethal phenotype of loss in expression. Taken together, the data presented here of SUG1 in yeast. We have examined the tissue distrisuggest that while m56 is a highly conserved gene that bution of m56 using Northern and Western blots, in presumably plays essential but complex roles in basal addition to immunocytochemistry and in situ hybridand developmental processes, it may not represent a ization. While m56 was expressed in all tissues and rate-limiting step in these processes. ᭧ 1997 John cells examined, several classes of neurons, most nota-