A method is described for identifying serine phosphorylation sites in proteins, based on conventional 32 P labeling followed by electrophoretic separation, 'in-gel' digestion with a protease, peptide extraction, reversed-phase high-performance liquid chromatographic separation and collection and off
β¦ LIBER β¦
Identification of a Novel Phosphorylation Site in Human Androgen Receptor by Mass Spectrometry
β Scribed by Zixin Zhu; Robert R. Becklin; Dominic M. Desiderio; James T. Dalton
- Book ID
- 115588099
- Publisher
- Elsevier Science
- Year
- 2001
- Tongue
- English
- Weight
- 208 KB
- Volume
- 284
- Category
- Article
- ISSN
- 0006-291X
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## Abstract Nephrin is a typeβ1 transmembrane glycoprotein and the first identified principal component of the glomerular filtration barrier. Ten potential asparagine (__N__)βlinked glycosylation sites have been predicted within the ectodomain of nephrin. However, it is not known which of these pot