Identification of a monoclonal antibody to abscission tissue that recognises xylose/fucose-containing N-linked oligosaccharides from higher plants
✍ Scribed by Michael T. McManus; Jane McKeating; David S. Secher; Daphne J. Osborne; David Ashford; Raymond A. Dwek; Thomas W. Rademacher
- Publisher
- Springer-Verlag
- Year
- 1988
- Tongue
- English
- Weight
- 991 KB
- Volume
- 175
- Category
- Article
- ISSN
- 0032-0935
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✦ Synopsis
Monoclonal antibodies raised against extracts of the rachis abscission zone of Sambucus nigra L. were selected for high reactivity towards abscission-zone proteins. One antibody (YZ1/2.23) has been shown to cross-react, by both indirect and competition enzyme-linked immunosorbent assay and by Western blotting, with a number of plant enzymes including horseradish peroxidase, rice ~-glucosidase, almond fi-glucosidase and the lectins from Phaseolus vulgaris and Erythrina cristagalli.
The major N-linked oligosaccharide isolated from horseradish peroxidase has the sequence Man~ 3(Man~ 6)(Xylfl 2)Manfi 4GlcNAcfi 4(Fuc~ 3) GlcNAc. This oligosaccharide was found to be a potent inhibitor of the binding of YZ1/2.23 to the intact glycoprotein. The common determinant is therefore contained within this structure.