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Identification of a Model Cardiac Glycoside Receptor: Comparisons with Na + ,K + -ATPase †

✍ Scribed by Kasturi, Rama; Yuan, Jie; McLean, Larry R.; Margolies, Michael N.; Ball, William J.


Book ID
127312956
Publisher
American Chemical Society
Year
1998
Tongue
English
Weight
115 KB
Volume
37
Category
Article
ISSN
0006-2960

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A method to measure high-affinity binding sites for fluorescent ligands is described. The method is applied to the determination of (Na,K)-ATPase using the fluorescent ouabain derivative anthroylouabain (P. A. G. Fortes (1977) Biochemistry 16, 531-540). The method consists of measurements of the flu