Identification of a human group C rotavirus in Malaysia

✍ Scribed by Nassar B. G. Rasool; M. Hamzah; M. Jegathesan; Y. H. Wong; Y. Qian; Kim Y. Green

• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 379 KB
• Volume: 43
• Category: Article
• ISSN: 0146-6615
• DOI: 10.1002/jmv.1890430302

✦ Synopsis

Abstract

Stool specimens from 334 infants and young children hospitalized with diarrhea in the General Hospital, Kuala Lumpur, Malaysia between August and November, 1987 were analyzed for the presence of rotavirus double‐stranded (ds) RNA by polyacrylamide gel electrophoresis. Of the 334 specimens analyzed, 32 (9.6%) were positive for rotavirus RNA. One specimen (designated G147) exhibited ads RNA electrophero‐type profile characteristic of Group C rotavirus and was selected for further characterization. In Northern blot hybridization studies, the gene 5 segment of strain G147 hybridized with a cDNA probe generated from the cloned gene 5 (which encodes the VP6 inner capsid protein that is group specific) of porcine Group C rotavirus strain Cowden, confirming the classification of strain G147 in Group C. The association of Group C rotavirus with diarrheal illness in Malaysia is consistent with earlier studies that suggest a global distribution of this virus and supports the need for additional epidemiologic studies. © 1994 Wiley‐Liss, Inc.