Identification of a gibberellin-induced gene in deepwater rice using differential display of mRNA

✍ Scribed by Esther Knaap; Hans Kende

• Book ID: 104615753
• Publisher: Springer
• Year: 1995
• Tongue: English
• Weight: 563 KB
• Volume: 28
• Category: Article
• ISSN: 0167-4412
• DOI: 10.1007/bf00020405

✦ Synopsis

Differential display of mRNA was employed to identify gibberellin (GA)-regulated genes in deepwater rice. One of the first differentially displayed products identified was shown to be ten-fold induced after start of GA treatment. The sequence of the clone shows complete amino acid identity with histone H3, and its increased mRNA level correlates with the onset of DNA synthesis. We also identified a gene whose expression pattern did not change over the course of treatment with GA and can be used as standard to correct for loading differences on northern blots.