Identification of a gene on human chromosome 8q11 that is differentially expressed during prostate-cancer progression
✍ Scribed by Glenn T.G. Chang; Nita Tapsi; Martine Steenbeek; Leen J. Blok; Wytske M. van Weerden; Dirk C.J.G. van Alewijk; Bert H.J. Eussen; Gert J. van Steenbrugge; Albert O. Brinkmann
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- French
- Weight
- 195 KB
- Volume
- 83
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Using differential-display RT-PCR analysis between androgen-dependent LNCaP-FGC and androgen-independent
LNCaP-LNO human prostate-cancer cells, we have identified a gene not previously described as being expressed in prostate. The gene is more highly expressed in androgenindependent than in androgen-dependent LNCaP prostatecancer cells. Sequence analysis showed that the gene has already been cloned as a transcript present in embryonic brain, with unknown functions. Expression of the gene was found not to be restricted to the prostate, and not regulated by androgens in androgen-independent prostate-cancer cells. In concert with the cell-culture system, Northern-blot analysis of gene expression in vivo, using a panel of human prostate-cancer xenografts, demonstrated that the gene is more highly expressed in androgen-independent than in androgen-dependent prostate-cancer xenografts. The gene could be mapped on human chromosome 8q11. The 8q arm is known to be frequently amplified during prostate-cancer progression and harbors several proto-oncogenes potentially involved in cancer development. Since expression of the gene is positively correlated with prostate-cancer progression and its 8q11 chromosomal localization, we hypothesize that the gene may be involved in the development and progression of prostate cancer.