Identification of a decapeptide with the binding reactivity for tumor-associated TAG72 antigen from a phage displayed library
✍ Scribed by Gui, Jin; Moyana, Terence; Malcolm, Bruce; Xiang, Jim
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 835 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0887-3585
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✦ Synopsis
Peptide ligands for tumor-associated TAG72 antigen were identified by screening a large, diverse decapeptide library expressed on the surface of filamentous phages. Fifty-eight clones of phages were selected from the eluates after the third round of biopanning and their DNA inserts were sequenced. A dominant decapeptide HYVSIELPDH (14/58) was found with the binding reactivity for TAG72 antigen in the TAG72-binding ELISA and Western dot blotting. It also showed a preferential binding to colonic adenocarcinomatous cells expressing the TAG72 antigen in the histochemical study. Therefore, this anti-TAG72 decapeptide may be useful in serving as the starting point with regard to further designing peptidomimetics for potential pharmaceuticals.