Identification of a cDNA clone specific for the oligodendrocyte-derived repulsive extracellular matrix molecule J1-160/180
✍ Scribed by B. Fuss; U. Pott; P. Fischer; M. E. Schwab; Dr. M. Schachner
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 927 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0360-4012
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✦ Synopsis
A cDNA clone specific for the oligodendrocyte-derived extracellular matrix glycoproteins J1-160/180 was obtained from a X ZAP11 expression library using polyclonal antibodies generated against mouse J1-160. The library was constructed from poly(A)+-RNA isolated from 0 1 antigen-positive rat oligodendrocytes. The cDNA clone expressed a fusion protein that was recognized by the J1-160/180-specific monoclonal antibodies 596, 619, and 620, and, weakly, 597. The fusion protein was not recognized by polyclonal antibodies to mouse Jlhenascin. The cDNA clone with an insert of approximately 5.6 kb in size contained the nucleotide sequence coding for the amino acid sequence of the N-terminus of a tryptic peptide derived from mouse 51-160. The developmental and tissue distribution of the mRNA recognized by the cDNA clone is in agreement with the described expression of the J1-160/180 proteins.