✦ LIBER ✦

Identification of a biochemical marker for the secretory pathway in Tetrahymena thermophila

✍ Scribed by Nita J. Maihle; Birgit H. Satir

Publisher: John Wiley and Sons
Year: 1986
Tongue: English
Weight: 525 KB
Volume: 31
Category: Article
ISSN: 0730-2312
DOI: 10.1002/jcb.240310302

No coin nor oath required. For personal study only.

✦ Synopsis

Tetrahymena themphila is a ciliated protozoan studied by investigators from a wide range of disciplines as a model system for a variety of specialized eukaryotic cell functions. The proteinaceous secretory products of T thermophila have been isolated and characterized [l] and in this study we identify the major secretory product, a 34,000 Mr polypeptide, and use an antiserum prepared against this secretory protein to (1) demonstrate that this 34,000 Mr polypeptide is truly a secreted protein in Tetruhymena and (2) monitor the synthesis and transport of this protein by indirect immunofluorescence and light microscopy during mucocyst biogenesis.

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