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Identification of 9, 10-epoxyoctadecanoic acid in human urine using gas chromatography-mass spectrometry

✍ Scribed by Gunnar A. Ulsaker; Gerd Teien

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
493 KB
Volume
9
Category
Article
ISSN
0269-3879

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✦ Synopsis

9,lO-epoxyoctadecanoic acid has been detected in human urine. Two simple purification procedures were used; the one based upon liquid-liquid extraction and the other based upon sorbent extraction technology isolating the free fatty acid fraction. Prior to trimethylsilylation and gas chromatographic-mass spectrometric analysis, both the epoxy and carhoxy functions were reduced to hydroxy groups. The shift in fragmentation of a deuterated sample verified the presence of intact epoxide prior to chemical reduction. Special attention was paid to the risk of false identification of the epoxide. The content of 9,lO-epoxyoctadecanoic acid in human urine was estimated to be 2.1 nM/L.

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