β Scribed by J. Dennis Mahuren; Thomas A. Pauly; Stephen P. Coburn
No coin nor oath required. For personal study only.
Plasma and urine from individuals ingesting large amounts of vitamin B 6 contain several unidentified vitamin B 6 metabolites. Based on movement in 6 TLC solvents, elution in HPLC, and ultraviolet and infrared spectra, two of these metabolites have been identified as 5-pyridoxic acid and its lactone. These two compounds can account for 10-20% of the urinary vitamin B 6 metabolites.
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Simultaneous and direct assays of pyridoxal, pyridoxal-5'-phosphate, and pyridoxic acid in human serum are described. The method applied is based on the reaction of these compounds with beryllium in an ammoniacal medium to yield highly fluorescent derivatives. Overlapping of conventional fluorescenc
Marginal deficiency of vitamin B6 has recently been related to cardiovascular diseases. Because of that there is an increasing interest in a suitable and reliable method for quantifying this vitamin in routine laboratory medicine. We have developed a HPLCbased method able to quantify the B6 vitamers