Identification of 17α, 20α-dihydroxyprogesterone in testicular extracts after incubation with ketoconazole

✍ Scribed by W. F. J. Lauwers; L. Le Jeune; H. Vanden Bossche; G. Willemsens

• Book ID: 101757678
• Publisher: John Wiley and Sons
• Year: 1985
• Tongue: English
• Weight: 404 KB
• Volume: 12
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/bms.1200120608

✦ Synopsis

The antifungal ketoconazole affects testosterone synthesis in dispersed rat testicular cells. In the presence of ketoconazole an accumulation of 17 alpha,20 alpha-dihydroxyprogesterone has been observed. This steroid was isolated from the testis of Wistar rats after a [4-14C]progesterone incorporation in the presence of ketoconazole. Its identification was achieved from the gas chromatographic/mass spectrometric analysis of the isolated radioactive fraction. A chemical derivatization of the fraction with butylboronic acid followed by mass spectrometric analysis confirmed the presence of 17 alpha,20 alpha-dihydroxyprogesterone.