Identification method for twelve oligomannose-type sugar chains thought to be processing intermediates of glycoproteins
✍ Scribed by Sumihiro Hase; Shunji Natsuka; Hisashi Oku; Tokuji Ikenaka
- Book ID
- 102628684
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 392 KB
- Volume
- 167
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A mixture of the pyridylamino (PA) derivatives of 12 oligomannose-type sugar chains was fractionated into five fractions (mannose5N-acetylglucosamine2-PA approximately mannose9N-acetylglucosamine2-PA) by size-fractionation HPLC with a MicroPak AX-5 column. Each fraction thus obtained was then analyzed by reversed-phase HPLC with a Cosmosil 5C18-P column. In this way, the 12 PA-oligomannose-type sugar chains were completely separated from each other. The method was used to identify the structures of oligomannose-type sugar chains of human C3, the third component of human complement.