Identification and validation of endogenous reference genes for biomarker expression profiling in abalone under endocrine disrupting chemicals (EDCs) exposure by quantitative real-time RT-PCR

Identification and Validation of Endogen

Identification and Validation of Endogenous Reference Genes for Expression Profiling of T Helper Cell Differentiation by Quantitative Real-Time RT-PCR

✍ H.K. Hamalainen; J.C. Tubman; S. Vikman; T. Kyrölä; E. Ylikoski; J.A. Warrington 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 88 KB

Real-time RT-PCR method was exploited to identify endogenous reference genes in differentiating human T helper cells. When using this technology in our experimental system, finding a set of genes whose mRNA expression levels would not change appeared to be very challenging. Our initial plan to use t