Identification and quantitative GLC determination of iproniazid in human urine

✍ Scribed by R. M. De Sagher; A. P. De Leenheer; A. E. Claeys

• Publisher: John Wiley and Sons
• Year: 1976
• Tongue: English
• Weight: 517 KB
• Volume: 65
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600650619

✦ Synopsis

A GLC method for the specific identification and quantitation of iproniazid, a potent monoamine oxidase inhibitor drug, is described. The free drug is isolated from urine samples by two-phase extraction reinforced with salting out. Identification and quantitative determination a t the microgram level are done on aliquots of the chloroform extract by GLC, using the 2-butyl analog as an internal standard. Iproniazid and potentially interfering compounds present in the extracts are also identified by GLC-mass spectrometry and TLC for supporting evidence of the GLC method's specificity.

Keyphrases 0 Iproniazid-GLC analysis, urine GLC-analysis, iproniazid in urine 0 Monoamine oxidase inhibitors-iproniazid, GLC analysis, urine 0 CNS stimulants-iproniazid, GLC analysis,