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Identification and quantitative analysis of p-hydroxybenzic acid and its esters using reversed- phase thin-layer chomatography

✍ Scribed by R. Rangone; C. Ambrosio

Publisher: Elsevier Science
Year: 1970
Tongue: English
Weight: 550 KB
Volume: 50
Category: Article
ISSN: 1873-3778
DOI: 10.1016/s0021-9673(00)97969-x

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✦ Synopsis

The $-hydroxybenzoic acid and its methyl, ethyl, propyl, butyl and benzyl esters are separated on thin layers of silanized silica gel using borate buffer (pH II) as mobile phase, adding a given volume of organic solvent as required. The separation of the various compounds is complete and an accurate quantitative determination of the #-hydroxybenzoic acid and its esters is possible using a simple technique of elution and spectrophotometric determination.

Pre$aYatio9z of the chromatogra$&y solutiorts Methanol solutions of pure commercial products (Merck, BDH, Carlo Erba) 1. C?WO?~ldO&, 50

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