Identification and production of flavonoids in a cell suspension culture ofScutellaria baicalensisG.

✍ Scribed by Weon Taek Seo; Young Hoon Park; Tae Boo Choe

• Publisher: Springer
• Year: 1993
• Tongue: English
• Weight: 390 KB
• Volume: 12-12
• Category: Article
• ISSN: 0721-7714
• DOI: 10.1007/bf00234703

✦ Synopsis

A high yielding cell line of Scutellaria baicalensis G. has successfully been developed to produce flavonoids. Major components of the flavonoids were identified as baicalin and wogonin-7-O-glucuronic acid by a series of instrumental analyses using UV, IR, MASS, and NMR. After 12 days in suspension culture, the cell growth reached 14 g (DW)/l, and baicalin and wogonin-7-O-glucuronic acid were obtained in concentrations of 2.9 g/l and 1.07 g/l, respectively. The culture temperature was found to be an important parameter for improving production yield of the flavonoids. The yield of baicalin was observed to increase to 4.2 g/l by shifting the temperature from 30 °C to 25 °C after 72 h of suspension culture.