Partial purification and characterizatio
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Roy R. Lobb; Marc E. Key; Edward M. Alderman; James W. Fett
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Article
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1985
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John Wiley and Sons
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French
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The established human colon adenocarcinoma cell line, HT-29, secretes a vascular permeability factor (VPF) in vitro. The factor has been purified from serum-free conditioned medium by acidification, cation-exchange, and reverse-phase and anion-exchange high-performance liquid chromatography. The VPF