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Identification and inter-relationship analysis ofBradyrhizobium japonicumstrains by restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD)

✍ Scribed by V. R. Lunge; N. Ikuta; A. S. K. Fonseca; D. Hirigoyen; M. Stoll; S. Bonatto; L. S. Ozaki

Publisher: Springer
Year: 1994
Tongue: English
Weight: 829 KB
Volume: 10
Category: Article
ISSN: 1573-0972
DOI: 10.1007/bf00327952

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✦ Synopsis

Genomic DNA of 13 Bradyrhizobium japonicum strains was prepared and analysed by restriction fragment length polymorphism (RFLP) with nif and nod probes, and by random amplified polymorphic DNA (RAPD) with 11 primers of arbitrary nucleotide sequence. Polymorphism was observed in both analyses. The RFLP and RAPD banding patterns of different strains were used to calculate genetic divergence and to construct phylogenetic trees, allowing studies on the relationships between the strains. RFLP with nif and nod probes permitted the separation of the strains into two divergent groups, whereas RAPD separated them into four main groups. RAPD allowed closely related strains to be distinguished.

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