Identification and expression of water stress- and abscisic acid-regulated genes in a drought-tolerant sunflower genotype
✍ Scribed by Olivier Ouvrard; Françoise Cellier; Karine Ferrare; Didier Tousch; Thierry Lamaze; Jean-Marc Dupuis; Francine Casse-Delbart
- Publisher
- Springer
- Year
- 1996
- Tongue
- English
- Weight
- 994 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0167-4412
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✦ Synopsis
We have studied two lines of sunflower (Helianthus annuus L.) selected in the field as drought-tolerant (RI genotype) or drought-sensitive (S 1 genotype). When subjected to drought conditions, the RI line was able to maintain high leaf water potential longer and wilted later than the S 1 line. Therefore, this indicates that RI tolerance includes a leafadaptive response. By subtractive hybridization, we have isolated six different cDNAs (designated sdi for sunflower drought-induced) corresponding to transcripts accumulated in R1 and S 1 leaves during adaptive response. Analysis of transcript accumulation in response to drought in both genotypes suggests a preferential expression of three sdi genes in the tolerant line. Abscisic acid-mediated induction, analysed in R1 leaves, was observed for only four sdi genes. Sequence analysis of six sdi clones revealed that five clones were related to known proteins including non specific lipid transfer proteins (nsLTP), early light-induced proteins (ELIP), l-aminocyclopropane-l-carboxylate oxidase (ACC oxidase) or dehydrins, predicted to be involved in a wide range of physiological processes.