Identification and expression of boar sperm proteins in the reproductive tract that interact with antibodies in serum from an infertile woman
✍ Scribed by Haneji, Tatsuji ;Nagano, Toshio ;Koide, S. S.
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 952 KB
- Volume
- 259
- Category
- Article
- ISSN
- 0022-104X
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✦ Synopsis
Abstract
Serum designated as IS obtained from a young healthy infertile woman induced a head‐to‐head agglutination of ejaculated boar sperm. The immunoglobulin G (IgG) prepared from IS localized to the acrosomal region of the sperm head obtained from the corpus and cauda epididymis as determined by an indirect immunofluorescent method. The IgG interacted with a boar sperm protein with an estimated molecular weight of 45‐kDa, determined by sodium dodecyl sulfate‐polyacrylamide gel electrophoretic (SDS‐PAGE) immunoblotting technique. However, the IgG did not interact with proteins extracted from sperm obtained from the testis and caput epididymis or from non‐gonadal tissues including liver, kidney, spleen, muscle and serum. The IgG interacted with additional proteins of about 75‐and 38‐kDa present in the corpus and cauda epididymal fluids but not those in the caput epidiymal fluid. The staining intensity of the 75‐kDa band was reduced and that of the 38‐kDa was nullified with ejaculated seminal plasma proteins. The interacting proteins were adsorbed when chromatographed on Concanavalin A Sepharose column, suggesting that they are glycoproteins.