Identification and determination of thyroxine using gas-liquid chromatography

✍ Scribed by Gerald J. Manius; Patricia Fallon; Ronald Tscherne

• Publisher: Elsevier Science
• Year: 1978
• Tongue: English
• Weight: 486 KB
• Volume: 87
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(78)90699-1

✦ Synopsis

A quantitative gas-liquid chromatographic assay method has been developed for the thyroid hormone thyroxine as a bulk substance. The trimethylsilylated derivative is chromatographed with an internal standard on an OV-101 column by means of flame ionization detection. Analogs of thyroxine can be identified and quantitated as possible contaminants. Electron-capture detection of thyroxine at the picogram level has been demonstrated. Complete silylation has been confirmed.

MATERIALS AND METHODS

Thyroxine (tetraiodothyronine)

reference standard material is USP grade and was obtained from U.S.P.C., Rockville, Md. In accord with label instructions it was dried under vacuum at 60°C for 4 hr prior to use.