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Identification and Cloning of a New Category of DNA Fragments Which Are Poorly Represented in Human Genomic Libraries

โœ Scribed by P. Wong; Y. Myal; R. Shui; M. Tenniswood


Book ID
115572458
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
711 KB
Volume
190
Category
Article
ISSN
0006-291X

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We previously reported an efficient method for subtractive cDNA cloning using oligo(dT) \()_{30}\)-Latex and polymerase chain reaction (PCR) (E. Hara et al., \(\mathrm{Nu}\) cleic Acids Res. 19, 7097-7104, 1991). The subtraction was performed by hybridization between mRNA of cell type \(B\) and the