𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Identification and classification of celery cultivars with RAPD markers

✍ Scribed by X. Yang; C. Quiros

Publisher
Springer
Year
1993
Tongue
English
Weight
684 KB
Volume
86-86
Category
Article
ISSN
0040-5752

No coin nor oath required. For personal study only.

✦ Synopsis

Twenty-one celery (Apium graveolens L. var. dulce) cultivars, one celeriac (var. rapaceum) and one annual smallage (var. secalinum) cultivar were screened for polymorphic RAPD (Random Amplified Polymorphic DNA) markers with 28 arbitrary 10-mer primers. Among a total of 309 bands observed, 29 (9.3%) were polymorphic in the 23 cultivars screened, but only 19 (6.1%) markers were polymorphic within the 21 type dulce cultivars. These markers were sufficient to distinguish each of the cultivars used. The average marker difference was 6.4 between two celery cultivars, 16.7 between celery and annual smallage, 14.7 between celery and celeriac, and 12.0 between annual smallage and celeriac. The celery cultivars surveyed were classified into three groups based on the marker differences. The relationship among the dulce-type cultivars concluded from this research is basically consistent with the known lineage of the cultivars and the previous study using stem protein and isozyme markers. RAPD technology provides a new alternative for cultivar identification and classification in celery.

πŸ“œ SIMILAR VOLUMES

Identification of broccoli and cauliflow
Identification of broccoli and cauliflower cultivars with RAPD markers
✍ Jinguo Hu; Carlos F. Quiros πŸ“‚ Article πŸ“… 1991 πŸ› Springer 🌐 English βš– 835 KB

RAPD (Random Amplified Polymorphic DNA) markers generated by 4 arbitrary 10-mer primers, discriminated 14 broccoli and 12 cauliflower cultivars (Brassica oleracea L.) by banding profiles. The size of the amplified DNA fragments ranged from 300 to 2600 base pairs. Twenty-eight percent of the markers

Identification of apple cultivars using
Identification of apple cultivars using RAPD markers
✍ B. Koller; A. Lehmann; J. M. McDermott; C. Gessler πŸ“‚ Article πŸ“… 1993 πŸ› Springer 🌐 English βš– 427 KB

Eleven apple cultivars were differentiated using randomly amplified polymorphic DNA (RAPD) markers obtained by the polymerase chain reaction (PCR). The variability of the technique and of the origin of the DNA extract was analyzed. A set of bands consistent in their presence or absence was chosen to

Genetic variation detected with RAPD mar
Genetic variation detected with RAPD markers among upland and lowland rice cultivars (Oryza sativaL.)
✍ L. -X. Yu; H. T. Nguyen πŸ“‚ Article πŸ“… 1994 πŸ› Springer 🌐 English βš– 586 KB

Genetic variation of nine upland and four lowland rice cultivars (Oryza sativa L.) was investigated at the DNA level using the randomly amplified polymorphic DNA (RAPD) method via the polymerase chain reaction (PCR). Forty-two random primers were used to amplify DNA segments and 260 PCR products wer