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Identification and characterization ofCEN12in the budding yeastSaccharomyces cerevisiae

✍ Scribed by Alison E. Gammie; Mark D. Rose

Publisher: Springer-Verlag
Year: 1995
Tongue: English
Weight: 422 KB
Volume: 28
Category: Article
ISSN: 0172-8083
DOI: 10.1007/bf00518162

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✦ Synopsis

In this paper we report the cloning, sequencing and functional characterization of CEN12 and an associated autonomously replicating sequence (ARS) from the budding yeast Saccharomyces cerevisiae. In the course of studying a dynamin-related gene, DNM1, we previously physically mapped the gene to chromosome 12. Genetic mapping showed that the gene was tightly linked (0.35 cM) to the centromere. Subcloning experiments revealed that a centromere-like activity was included in a small segment of DNA immediately downstream from the DNM1 gene. Mitotic centromere activity was discerned by the ability of the region to de-stabilize a centromere-containing plasmid, and to stabilize an ARS-containing plasmid. Meiotic centromere activity was determined by the first-division segregation in crosses of ARS plasmids containing this region. The DNA sequence of this region revealed a sequence with strong homology to the consensus for yeast centromeres.

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